control cdna sample  (Promega)

Journal: The Journal of Clinical Investigation

Article Title: BCL-W has a fundamental role in B cell survival and lymphomagenesis

doi: 10.1172/JCI89486

Figure Lengend Snippet: (A) qRT-PCR analysis (in triplicate) of samples from patients with BL (n = 15) compared with normal lymphoid control tissue (n = 7). mRNA levels were normalized to β-actin and presented as 2–ΔΔCt. Bars represent the mean ± SEM. *P = 9.1 × 10–4, by t test. (B) Microarray gene expression–profiling data for BCL-W and BCL-2 mRNA in BL (n = 57) compared with normal B cells (n = 49). Bars represent the mean ± SEM. Each circle represents 1 sample, and the y axis represents normalized expression of BCL-W and BCL-2. *P = 1.8 × 10–8, by t test. The data sets analyzed are listed in Supplemental Table 1. (C) Immunohistochemical analysis for BCL-W and BCL-2 protein was performed on samples from patients with BL (n = 26). Box and whisker plots of pathologist scores and representative images of BCL-W and BCL-2 staining from the same tumor sample are shown. Original magnification, ×40; scale bars: 200 μm. *P < 0.0001, by t test. For the box and whisker plots, the box represents the 25th and 75th percentiles, the line indicates the median, the circle indicates the mean, and the whiskers represent the maximum and minimum. (D) Protein expression was assessed by Western blotting for the indicated proteins in BL and DLBCL cell lines. Controls included tissue from 2 human spleens and purified B cells from human peripheral blood.

Article Snippet: For qRT-PCR analysis of DLBCL, TissueScan cDNA arrays containing 26 samples from patients with DLBCL and 10 normal lymphoid tissue controls were obtained from OriGene.

Techniques: Quantitative RT-PCR, Microarray, Expressing, Immunohistochemical staining, Whisker Assay, Staining, Western Blot, Purification

Journal: The Journal of Clinical Investigation

Article Title: BCL-W has a fundamental role in B cell survival and lymphomagenesis

doi: 10.1172/JCI89486

Figure Lengend Snippet: (A) Immunohistochemical analysis for BCL-W and BCL-2 protein was performed on samples from patients with DLBCL (n = 57). Box and whisker plots of pathologist scores and representative images of BCL-W and BCL-2 staining from the same 3 tumor samples. Original magnification, ×40; scale bars: 200 μm. For the box and whisker plots, the box represents the 25th and 75th percentiles, the line indicates the median, the circle indicates the mean, and the whiskers represent the maximum and minimum. (B) qRT-PCR analysis in triplicate of samples from patients with DLBCL (n = 26) compared with normal lymphoid control tissue (n = 10). mRNA levels were normalized to β-actin and are presented as 2–ΔΔCt. Error bars represent the mean ± SEM. **P < 6.03 × 10–5 and *P = 0.014, by t test. (C) Microarray gene expression–profiling data for BCL-W and BCL-2 mRNA in DLBCL (n = 319) compared with normal B cells (n = 49). Lines represent the mean ± SEM. Each circle represents 1 sample, and the y axis represents normalized expression of BCL-W and BCL-2. **P < 2.2 × 10–16 and *P = 9.96 × 10–5, by t test. The data sets analyzed are listed in Supplemental Table 1. (D) Patient samples of DLBCL with low BCL-2 from the GSE31312 and GSE10846 data sets were each separated into 2 groups (BCL-W high and BCL-W low) on the basis of the median expression of BCL-W, and Kaplan-Meier analyses were performed. The P values in D were determined by log-rank test.

Article Snippet: For qRT-PCR analysis of DLBCL, TissueScan cDNA arrays containing 26 samples from patients with DLBCL and 10 normal lymphoid tissue controls were obtained from OriGene.

Techniques: Immunohistochemical staining, Whisker Assay, Staining, Quantitative RT-PCR, Microarray, Expressing